Pig / 5-12 Weeks / Female / Not pregnant / Hygiene status: Conventional Incident The incident happened in the field of Anesthesia during Experiment/Experiment set-up/Evaluation. Background information Anaesthesia for acute laparotomy of a pig was induced by intramuscular injection of ketamine 10mg/kg and midazolame 0.5mg/kg as well as atropine 0.02mg/kg, the pig was brought to surgery room. Here a venous catheter was placed in ear vene, thiopental 10mg/kg was given intravenously before orotracheal intubation. Isoflurane in oxygen and air should be given to maintain anaesthesia with pressure controlled breathing as well as analgesia by fentanyl. Negative experience Directly after starting to maintain general anaesthesia with inhalation of isoflurane (2 Vol.%) in oxygen and air, the carbogene decreased from 45% to values <10% in exhalation air. Disturbances in ECG were seen, heartrate increased from 120/min up to 180/min. Isoflurane was dropped, only oxygen was given. It was not possible to stabilize the animal. The animal was inhaled manually via Ambotube with oxygen. After a few seconds only atrial flickering was seen. Intravenously given adrenaline had no effect. Intubation was controlled, without any negative result. The animal died without recovering from anaesthesia. Theacute laparotomy could be conducted in dead animal, but no realistic situation accompanied by blood filled vessels a.s.o. was provided anymore. Where do you see the reason(s) for the incident and how could they be avoided in the future? Because of no other side effects we assume the pig developed a lung emboly - seen be suddenly dropped carbogen concentration in exhalation air as well as atrial flickering and no chance of stabilizing the animal. The reason is not known. A post mortal examination was not conducted. For any further anaesthesia heparine will be stored for emergency cases. Assessment of the degree of distress for the animal Mild(1) Are the results of the experiment usable? Significant impairment How often does an incident like this approximately occur? First time Which factors contributed to the occurence of the incident? Animal factors (restriction, med. condition, etc.) ------------------------------------------------------------- The reporting person is from Researcher within the project
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